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The surface structure of murine ascites tumors. II. Difference in cytotoxicity of various phytoagglutinins toward yoshida sarcoma cells in vitro
Author(s) -
Tomita Motowo,
Kurokawa Tsutoma,
Onozaki Kikuo,
Osawa Toshiaki,
Sakurai Yoshio,
Ukita Tyunosin
Publication year - 1972
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910100319
Subject(s) - cytotoxicity , abrus precatorius , galactose , agglutinin , in vitro , biology , biochemistry , ricinus , cell , cytotoxic t cell , sugar , lectin , botany
Several phytoagglutinins having different sugar specificities were found to exert different agglutinating activity toward murine ascites cell lines. It was also found that some of these phytoagglutinins, especially. Ricinus communis. and. Abrus precatorius. agglutinins, inhibited the growth of Yoshida sarcoma (YS) cells and showed a strong cytotoxic effect. This kind of cytotoxicity of. Ricinus communis. agglutinin, which is specific for galactose, was shown to be counteracted by the addition of galactose but not of glucose, and this fact indicated that the cytotoxicity of the phytoagglutinin is related to the binding specificity of the agglutinin toward the sugar chains on the cell surface. On the other hand, in some phytoagglutinins, the cytotoxicity was found to be relatively low even when their agglutinating activity was remarkably high. On the basis of these results, the role of the sugar chains on cell surface in cellular functions is discussed.