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The SV40 S antigen; A carcinoembryonic‐type antigen of the hamster?
Author(s) -
Berman Leonard D.
Publication year - 1972
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910100213
Subject(s) - hamster , antiserum , heterologous , baby hamster kidney cell , biology , antigen , microbiology and biotechnology , cell culture , embryo , carcinoembryonic antigen , virology , antibody , somatic cell , mesocricetus , virus , immunology , cancer , genetics , gene
In addition to hamster tumor cells induced or transformed by SV40, hamster anti‐SV40 S (Surface) antiserum also reacted with non‐SV40‐exposed cell lines spontaneously induced (BHK 21) or transformed by heterologous oncogenic DNA and RNA viruses in the indirect membrane fluorescent antibody test. The antiserum titered equally with both BHK and SV40‐transformed cells and the reaction could be absorbed with either of these cell lines, or with hamster embryo. The antiserum also reacted with early hamster embryo cells, although various organ cells from late fetuses, newborns and adults were negative. Mouse cell lines spontaneously induced (BALB/c‐3T3) or transformed by SV40 and adenovirus 12 were also non‐reactive. The results suggest that the antigen in question is a hamster somatic antigen present during embryonic life and derepressed in lines of actively growing cells.