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Chromosomal mechanism for the induction of reversion in transformed cells
Author(s) -
BlochShtacher Navah,
Rabinowitz Zelig,
Sachs Leo
Publication year - 1972
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910090321
Subject(s) - reversion , polyploid , ploidy , biology , aneuploidy , chromosome , karyotype , genetics , microbiology and biotechnology , cell , phenotype , gene
The changes in chromosome constitution that occur during induction of reversion in transformed cells have been studied. The culture of transformed cells at low cell density without a feeder layer, which induces reversion, also induced a change in chromosome number from diploid to aneuploid. The percentage of revertants formed under these conditions was related to the percentage of aneuploid cells. Most of the aneuploid cells had a subtetraploid chromosome number. The results indicate that the formation of aneuploids is due to the formation from binucleate cells of polyploid cells which later lose chromosomes, and suggest that conditions that stabilize the reverted state produce the right type of stable aneuploid cells required for reversion.