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Identity of the soluble ebv‐associated antigens of human lymphoid cell lines
Author(s) -
Reedman Beverley M.,
Pope J. H.,
Moss D. J.
Publication year - 1972
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910090120
Subject(s) - antigen , complement fixation test , cell culture , precipitin , biology , immunodiffusion , microbiology and biotechnology , pan t antigens , immunofluorescence , antibody , virus , virology , immunology , monoclonal antibody , serology , genetics
The reactions of human sera with antigens prepared from five human lymphoid cell lines were studied by immunodiffusion. Two major precipitation lines occurred with QIMR‐WIL antigens; one (S line) due to a soluble antigen and the other (F line) due to a sedimentable antigen. A close association in human sera of precipitins producing the S line with antibody to Epstein‐Barr virus (EBV) detectable by immunofluorescence (Henle test) and by complement fixation (CF) tests indicated a specific association of the S line with EBV, but the nature of the F line was not clarified. Soluble antigens prepared from five lymphoid cell lines (including Raji) contained at least one common identical heat‐resistant component, defined as the antigen giving the S line. The ease of detection of the S antigen in cell lines by immunodiffusion appeared to be related to the titre of the soluble EBV‐associated CF antigen, and the evidence suggested that the soluble CF and ID antigens were probably identical.