Premium
The topography of some anionic sites at the surfaces of fixed ehrlich ascites tumour cells
Author(s) -
Weiss L.,
Jung O. S.,
Zeigel R.
Publication year - 1972
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910090107
Subject(s) - neuraminidase , glutaraldehyde , ribonuclease , rnase p , colloid , biophysics , chemistry , biology , enzyme , biochemistry , rna , chromatography , gene
The distribution of positively charged, colloidal iron hydroxide particles to the surfaces of glutaraldehyde‐fixed Ehrlich ascites tumour cells, was studied in 700Å thick sections. After incubation with neuraminidase and/or ribonuclease, and fixation, cells were also reacted with particles. It was shown that the numbers of bound particles were significantly reduced by enzyme treatment, which had previously been shown to reduce the densities of anionic sites at the cell surfaces. Statistical analyses showed that in the controls and ribonuclease‐treated cells, the particles were adsorbed in surface locations following a Poisson distribution. Following neuraminidase treatment, the particles were arranged in clusters, but the clusters themselves were arranged in a Poisson distribution. The data are tentatively interpreted as indicating the presence of different sized neuraminidase‐ and ribonuclease‐susceptible zones at the cell surface, having a higher than average density of anionic sites.