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Inhibition of hipa plasmacytoma cell‐free transmission by normal spleen of BALB/c, DBA/2 and C3H mice
Author(s) -
Pedio G.,
Rüttner J. R.
Publication year - 1971
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910080318
Subject(s) - spleen , plasmacytoma , transplantation , balb/c , immune system , immunology , biology , cell , microbiology and biotechnology , andrology , medicine , multiple myeloma , biochemistry
Spleen from normal BALB/c, DBA/2 and C3H mice exerted a protective effect against syngeneic and allogeneic cell‐free transmission of HIPA plasmacytoma in mice. The maximal protective effect of these spleens was found in C3H and ICR mice (100‐70%). Inoculation of BALB/c spleen homogenates in syngeneic mice resulted in protection of about 56% of the cases. Spleen homogenates from BALB/c mice were not effective against cellular transplantation of HIPA plasmacytoma. The fact that the protection required one week to develop suggested that an immune reaction was involved. The factor responsible for protection was found in the sedimentable fraction of spleen homogenates centrifuged at 500 × g during 15 minutes and was destroyed by heat and freezing/thawing.