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Lymphocyte transformation in patients with Kaposi's sarcoma
Author(s) -
Taylor John F.,
Junge Ulrich,
Wolfe Lauren,
Deinhardt Friedrich,
Kyalwazi Sebastian K.
Publication year - 1971
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910080315
Subject(s) - sarcoma , lymphocyte , pathology , mitomycin c , homologous chromosome , in vitro , kaposi's sarcoma , malignant transformation , medicine , biology , immunology , surgery , biochemistry , human herpesvirus , gene
This study describes a correlation between the morphologic types of cutaneous Kaposi's sarcomata and the capacity of lymphocytes to be stimulated in culture with PHA and tumour cells. Lymphocytes from patients with the sarcoma and from normal controls were cultured in vitro with PHA, autologous Mitomycin‐inhibited tumour cells, and control cells. Lymphocyte transformation was evaluated by measuring the uptake of 125 iodo‐2′ deoxyuridine. Lymphocytes from seven of 21 Kaposi patients showed increased transformation in cultures containing tumour cells and six of these seven patients had nodular or infiltrative disease. An impaired response to both PHA and tumour cells was noted in patients with florid tumours, a form thought to be associated with a diminished host response. Standardized extracts of tumour and control cells were incubated with homologous lymphocytes from other Kaposi patients and normal controls. Two of nine cultures from Kaposi patients showed increased isotope uptake, compared with none of three control cultures.