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Immunofluorescent detection of murine and hamster C‐type virus species‐specific (gs‐1) determinants by monospecific guinea‐pig sera and interspecies‐specific (gs‐3) determinants by tumor‐bearing rat sera
Author(s) -
Hampar Berge,
Gilden Raymond V.,
Kelloff Gary,
Oroszlan Stephen,
Simms Donald
Publication year - 1971
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910080310
Subject(s) - hamster , biology , antiserum , cytoplasm , microbiology and biotechnology , guinea pig , immunofluorescence , virus , staining , virology , cell culture , antigen , antibody , immunology , biochemistry , genetics
Abstract The species‐specific (gs‐1) determinants of murine and hamster C‐type viruses were detected by direct immunofluorescence (FA) in fixed virus‐infected cells using monospecific guinea‐pig antisera. The interspecies‐specific (gs‐3) determinants were also detected by FA using broad spectrum sera from tumor‐bearing rats. The FA staining of gs‐1 and gs‐3 determinants in fixed infected cells was localized in the cytoplasm as punctate foci at or near the cell membrane and as a diffuse mass at the perinuclear region. The FA staining of live infected cells was limited to antisera with envelope activity. The percentage of FA positive cells in any one cell preparation was inversely related to the efficiency of complete virus synthesis. Blocking experiments support the contention that the gs‐1 and gs‐3 determinants reside on the same molecule with the former occupying a more accessible or dominant position.