Premium
Mechanism of resistance of yoshida sarcoma to nitrogen mustard
Author(s) -
Inaba Makoto,
Sakurai Yoshio
Publication year - 1971
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910070308
Subject(s) - nitrogen mustard , incubation , in vitro , drug resistance , drug , cell culture , membrane , cell , pharmacology , biology , chemistry , biochemistry , chemotherapy , genetics , cyclophosphamide
Abstract Uptake and binding of methyl‐bis(2‐chloroethyl)amine hydrochloride (HN 2 ) to the cells of Yoshida sarcoma during their acquisition of resistance to this drug was investigated. With the increase of resistance index, drug uptake by the cells decreased. A good parallelism was found between resistance index and binding rate of the drug with cellular constituents when the intact cells were kept contacted in vitro with the drug in an incubation medium. When the cells were homogenized before contact with the drug, there was no difference in binding rate of the drug between sensitive and resistant cell lines. Suppression of cell membrane transport seemed to play a role in the acquisition of resistance by the cells.