Premium
Further manifestations of abortive transformation of bhk 21 cells by polyoma virus
Author(s) -
TaylorPapadimitriou Joyce,
Stoker M. G. P.,
Riddle P.
Publication year - 1971
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910070211
Subject(s) - polyoma virus , virology , virus , transformation (genetics) , dna synthesis , dna , biology , mitosis , capsid , microbiology and biotechnology , genetics , gene
Abortive transformation of layer cultures of BHK 21 cells by polyoma virus has been studied using serum‐depleted medium. High doses of partially purified infectious virus, in the absence of serum, stimulate the majority of the cells to synthesize DNA while empty capsids do not. The onset of DNA synthesis occurs approximately 28 h after infection, and in medium containing 0.8% γ‐depleted serum, can be followed by one round of mitoses. A temporary loss of oriented arrangement of cells is also observed commencing 25 to 30 h after infection. Abortively transformed cells resemble stably transformed lines of BHK 21 cells in their requirements for the factors in serum operative in the control of growth.