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A dilute agar colony inhibition (CI) test for studying cell‐mediated immune responses against tumor cells
Author(s) -
Heppner G. H.,
Kopp J. S.
Publication year - 1971
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910070104
Subject(s) - immune system , antigen , biology , lymphocyte , incubation , agar , cell , immunity , cell mediated immunity , neoplasm , immunology , tumor cells , microbiology and biotechnology , cancer research , biochemistry , bacteria , genetics
A modification of the Hellström method for measuring cell‐mediated immunity to tumor‐associated antigens is described using mouse mammary tumors as a model. Target tumor cells are incubated with lymphocytes for 1 h and then cloned by the dilute agar method. The number of surviving cell colonies following incubation with immune lymphocytes is compared to the number of surviving tumor cell colonies incubated with control lymphocytes. Significant inhibition is obtained with as few as 10 immune lymphocytes for every target cell. Serum from immune animals can be shown capable of blocking lymphocyte‐mediated inhibition of tumor colony formation. The data obtained with this method are very similar to those obtained with the Hellström test. The advantages and disadvantages of the modification, as compared to the Hellström test, are discussed.