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Demonstration by colony inhibition methods of cellular and humoral immune reactions to tumour‐specific antigens associated with aminoazo‐dye‐induced rat hepatomas
Author(s) -
Baldwin R. W.,
Embleton M. J.
Publication year - 1971
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910070103
Subject(s) - lymph node , antigen , immune system , antigenicity , in vitro , immunofluorescence , immunology , immunization , biology , lymph , chemistry , antibody , pathology , medicine , biochemistry
Cellular and humoral immune reactions against tumour‐specific antigens on aminoazo‐dye‐induced rat hepatomas were demonstrated in vitro by the colony inhibition technique. Lymph‐node cells from syngeneic rats immunized against individual hepatomas inhibited colony formation by cells of the immunizing tumour compared with the effects of normal lymph‐node cells. Also immune lymph‐node cells were not inhibitory to hepatomas other than the one used for immunization, reflecting the individual specificity of the antigens on these hepatomas. Serum‐mediated inhibition of colony formation by rat hepatoma cells, in the presence of complement, was also demonstrated and these reactions were similarly directed against cells of the immunizing tumour. This specificity of colony inhibition by immune lymph‐node cells and serum further amplifies the evidence for the individually distinct antigenicity of these hepatomas already revealed by transplantation and immunofluorescence tests.