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Binding of dimethylaminoazobenzene metabolites to DNA and proteins. I. in vitro studies on a microsomal dependent system
Author(s) -
Meunier Michèle,
Chauveau Jean
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910060317
Subject(s) - microsome , dna , in vitro , carcinogen , biochemistry , enzyme , chemistry , covalent bond , covalent binding , aniline , methylcholanthrene , organic chemistry
Abstract Incubating dimethylaminoazobenzene [aniline ring (U) 14 C] (DAB), purified thymus DNA and rat liver microsomes led to a covalent binding of radioactivity to DNA and proteins, which implied the presence of bound DAB metabolites. This binding required an enzymatic activation. We studied some characteristics of the enzymatic process, i.e. NADPH requirement, effect of pH, effect of various concentrations of microsomes and DNA, induction by 3‐methylcholanthrene. This in vitro system is discussed in view of its validity for studying interactions between the carcinogenic azodyes and the cellular constituents.