Premium
Detectioni and isolatinio of tumour‐specific antigen associated with a spontaneously arising rat mammary carcinoma
Author(s) -
Baldwin R. W.,
Embleton M. J.
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910060307
Subject(s) - antigen , antigenicity , antibody , transplantation , immunofluorescence , biology , immune system , carcinoma , cell , mammary gland , pathology , cancer research , immunology , medicine , cancer , breast cancer , genetics
Cell surface expressed tumour‐specific antigen was detected in one (Sp4) of eight spontaneously arising rat mammary carcinomas by membrane immnofluorescence tests with viable tumour cells in suspensioni and sera from syngeneic rats immunized against each tumour. These findings are consistent with the frequency of expression of significant tumour antigenicity as defined by transplantation methods. Also the tumour antigen detected by membrane immunofluorescence tests in mammary carcinoma Sp4 showed the individual characteristics as the tumour‐specific transplantation antigens, and no evidence was obtained of weak common antigens in these tumours. Tumour‐specific antigen in subcellular fractions of mammary carcinomas Sp4 was assayed by its capacity to absorb antibody from tumour‐immune sera. By this criterion, it was established that plasma membrane fractionis retained tumour‐specific antigen although recoveries were low, amounting to less than 5% of cell‐surface experssed antigen. In comparison, almost total recovery was obtained of alloontigen experessed on mammary carcinoma Sp4 cells. Plasma membrane fractioins from mommary carcinoma Sp4 lacked the capacity to induce tumour rejectino reactions in syngeneic rats although a weak humoral antibody response was obtained. These findings contrast with the more consistent immune response elicted by heavily irradiated mammary carcinoa Sp4 cells.