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Immunological studies on mouse mammary tumors. V. Chemical analysis of further purified tumorspecific antigen of mouse mammary tumor
Author(s) -
Irie Reiko Furuse,
Kataoka Tateshi,
Mitsui Hiromi
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910060218
Subject(s) - sphingomyelin , antigen , phospholipid , antiserum , biochemistry , mammary tumor , biology , chemistry , mammary gland , biological activity , microbiology and biotechnology , membrane , in vitro , immunology , cancer , genetics , breast cancer
The tumor‐specific antigen present in mouse mammary tumor MM102 was further purified by 75% ethanol (EtOH Ag fraction) and its biological activity and chemical composition were analysed. Recovery of the antigenic activity was 25%, and the antigenic activity was found to be purified 22‐fold on a dry‐weight basis. This fraction inhibited the cytotoxic activity of the tumor‐specific antiserum (RMS), and induced heightened resistance against MM102 in syngeneic C3H/He mice. Chemical analysis of the lyophilized EtOH Ag fraction showed that it was composed of 11.7% protein, 83.3% lipid, 0.35% sugar, and 3.9% RNA. Lipid analysis showed that the ratio between phospholipid and neutral lipid in lipid moiety was 44:56 and the content of sphingomyelin in phospholipid was 19.3%. On the basis of these lipid analyses the possibility that the antigenic material might be extracted from plasma membrane is discussed.