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Effect of metabolic inhibitors on membrane immunofluorescence reactivity of established burkitt lymphoma cell lines
Author(s) -
Yata J.,
Klein G.,
Hewetson J.,
Gergely L.
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910050314
Subject(s) - antigen , cell culture , immunofluorescence , microbiology and biotechnology , biology , burkitt's lymphoma , puromycin , antibody , immunology , protein biosynthesis , genetics
Abstract The expression of the EBV‐associated membrane antigen complex was studied in three lines of cultured Burkitt lymphoma (BL) cells after exposure to various metabolic inhibitors and X‐irradiation. Direct membrane immunofluorescence (MIF) with a BL reference conjugate (F‐Mutua) revealed a high percentage of positive cells in two of the cell lines (Maku and Onesmas), provided they were cultured under conditions previously defined as optimal for the expression of the membrane antigen. The third cell line (Silfere) contained less than 5% MIF‐positive cells regardless of the culture conditions. Mitomycin C, actinomycin D, methotrexate and X‐irradiation increased the frequency of positive cells in Maku and Onesmas cultures kept under conditions favoring a low membrane antigen expression. Puromycin decreased the frequency of MIF‐positive cells in highly positive cultures from 50% to less than 10%. The increase or decrease in MIF became apparent 3 days after application of the inhibitor. Cytosine arabinoside and low temperature (31°C and 28°C) had no effect on MIF in cultures with either high or low membrane antigen expression, although cell growth was suppressed. In the Silfere cell line, none of the inhibitors studied had any effect on the expression of the membrane antigens.