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Carcinogenesis by RNA sarcoma viruses. XIV. Infection of stationary cultures with murine sarcoma virus (harvey)
Author(s) -
Murray R. K.,
Temin H. M.
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910050304
Subject(s) - virus , carcinogenesis , biology , sarcoma , virology , rna , cell culture , dna , embryo , cell division , cell , viral transformation , cancer , gene , genetics , pathology , medicine
No focus‐forming virus was produced after exposure of stationary mouse embryo cell cultures (achieved by removal of serum) to the marine sarcoma virus (Harvey) complex. Addition of serum to these infected stationary cultures resulted in MSV (Harvey) production following cell division. Cytosine arabinoside, an inhibitor of DNA synthesis, was found to inhibit subsequent virus production when added to stationary cultures immediately after exposure to virus. These results are compatible with a requirement for both cell division and non‐S‐phase DNA synthesis for the production of this virus complex, and show a marked parallelism to results previously obtained with the avian sarcoma complex.