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Altered glucose transport kinetics in murine sarcoma virus‐transformed BALB/3T3 clones
Author(s) -
Hatanaka Masakazu,
Todaro George J.,
Gilden Raymond V.
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910050209
Subject(s) - virus , biology , cell culture , 3t3 cells , murine leukemia virus , clone (java method) , virology , viral transformation , microbiology and biotechnology , embryo , sarcoma , neoplasm , leukemia , transfection , immunology , genetics , gene , pathology , medicine
The kinetics of glucose transport were determined for a number of spontaneous and virus‐transformed cell lines derived from a single clone of BALB/3T3 cells. SV40‐transformed and mouse leukemia virus‐infected cells were characterized by a Km very similar to that of the parental cell line. In contrast, murine sarcoma virus‐transformed cells had a much lower Km. These findings are in agreement with previous results obtained with mass cultures of NIH Swiss mouse embryo cells. One X‐irradiation and two spontaneous transformants also showed low Km values and no virions or virion antigens. These latter cell lines should provide an opportunity for a crucial test of the relationship between glucose transport kinetics and a functional sarcoma virus genome.