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Detection of specific surface antigens by colony inhibition in cells transformed by papovavirus SV40
Author(s) -
Tevethia Satvir S.,
Crouch Norman A.,
Melnick Joseph L.,
Rapp Fred
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910050203
Subject(s) - papovavirus , immunofluorescence , antigen , hamster , antiserum , biology , microbiology and biotechnology , antibody , in vitro , virology , complement fixation test , immunology , serology , biochemistry
Specific antigens at the surface of SV40‐transformed hamster cells were demonstrated by the in vitro colony inhibition and immunofluorescence tests. Antisera were prepared by inoculating hamsters either with purified SV40 or with human or marmoset SV40‐transformed cells. The inhibition of colony formation by the transformed cells ranged from 46 to 100% in the presence of specific antibody and complement. Nontransformed hamster cells were not inhibited by the immune sera. Of the 22 sera tested, 12 were positive in the colony inhibition test and 14 were positive in the immunofluorescence test. Eleven of the 22 sera tested were positive in both tests. This positive correlation between the colony inhibition and immunofluorescence tests suggests that the two tests are detecting the same or similar antigens.