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The distribution of the mouse mammary tumor agent and its antigenicity in milk
Author(s) -
Miroff George,
Lamberson Harold V.
Publication year - 1970
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910050118
Subject(s) - antigenicity , ficoll , immunogen , size exclusion chromatography , titer , chromatography , chemistry , antigen , pronase , density gradient , microbiology and biotechnology , biology , in vitro , biochemistry , immunology , virus , antibody , enzyme , peripheral blood mononuclear cell , physics , quantum mechanics , monoclonal antibody , trypsin
Inbred mice with a propensity for mammary tumor development contain the mammary tumor agent and a specific soluble antigen in their milk. These were found in approximately equal proportions in both the supernatant and the pellet after ultracentrifugation. Over 90% of the agent and the highest antigen titer were located in a density range of 1.06–1.075 in ficoll density gradients. The low tumorigenic potential and the antigenicity of the B particle band (p = 1.12) remained with the particles after removal of proteinaceous contaminant by gel filtration. The immunogen from the upper portion (p = 1.06–1.075) of ficoll gradients appears to aggregate when concentrated and eluted from gel filtration columns. The tumorigenic material isolated from ECTEOLA and carboxymethylcellulose chromatographic columns does not show a high positive correlation with the distribution of the virus‐like B particles, which was consistent with the finding that the agent was concentrated in a narrow region with an average density of 1.28 in cesium chloride density gradients. The antigen associated with B particles and the lower molecular weight active component are immunologically identical.