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Murine sarcoma virus: Titration patterns and interference by a murine leukemia virus
Author(s) -
Somers K. D.,
Kirsten W. H.
Publication year - 1969
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910040516
Subject(s) - virus , virology , murine leukemia virus , titration , viral interference , leukemia , biology , cell culture , microbiology and biotechnology , chemistry , immunology , viral replication , genetics , inorganic chemistry
Abstract A murine sarcoma virus (MSV‐K) was assayed by conversion of 3T3 mouse cells into foci of morphologically altered cells. Murine erythroblastosis virus (MEV), a member of the murine leukemia virus group, induced resistance in 3T3 cultures specific to super‐infection with focus‐forming MSV‐K. Interference by MEV was first detected at 14 days and reached maximal levels 21 days post infection. MSV‐K preparations from transformed 3T3 cells contained competent virions, as judged by “one‐hit” titration patterns. Since excess “helper” MEV in MSV‐K stocks could effectively convert titration patterns to “one‐hit” kinetics, the ratio of MEV to MSV‐K was determined by interference experiments. The ratio of MEV to MSV was approximately 10:1. Thus it appears that a single particle is capable of producing a focus of altered cells in the absence of simultaneous infection with a separate particle of MEV.