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A spleen weight assay for murine sarcoma virus harvey (MSV‐H)
Author(s) -
Hirsch Martin S.,
Harvey Jennifer J.
Publication year - 1969
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910040409
Subject(s) - weanling , spleen , virus , sarcoma , medicine , virology , antibody , immunology , biology , pathology
A spleen weight assay has been developed for titrating murine sarcoma virus‐Harvey (MSV‐H) 3 weeks after intraperitoneal infection of newborn BALB/c and random‐bred albino mice. Induction of splenomegaly corresponded in time with development of sarcomas and anaemia. The assay was also used to evaluate sera for neutralizing antibodies to MSV‐H. Weanling mice were much less susceptible to erythroblastic splenomegaly and tumour induction than newborns. Random‐bred mice were found more satisfactory for the assays than BALB/c mice. Newborn Sprague‐Dawley rats also responded to MSV‐H inoculation with a dose‐dependent erythroblastic splenomegaly.