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Immunology of 2‐acetylaminofluorene‐induced rat mammary adenocarcinomas
Author(s) -
Baldwin R. W.,
Embleton M. J.
Publication year - 1969
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910040107
Subject(s) - 2 acetylaminofluorene , antiserum , antigen , transplantation , neoplasm , immunofluorescence , adenocarcinoma , biology , immunity , cancer research , pathology , medicine , immunology , cancer , antibody , immune system , in vitro , biochemistry , microsome
The immunogenicities of 11 rat mammary adenocarcinomas induced with 2‐acetylaminofluorene (AAF) have been studied. Attempts were made to induce tumour‐specific immunity in syngeneic female rats, following treatment with heavily irradiated tumour or surgical removal of developing subcutaneous tumour grafts. Resistance was evoked against only two tumours and this was extremely weak. With the others, no resistance against the immunizing tumour could be demonstrated, even though the tests were made highly sensitive by the use of inocula of as few as 10 3 cells in pre‐irradiated hosts. Sera from rats immunized against six of the tumours were tested against viable cells of the immunizing tumour in an immunofluorescence test. In no case did tumour antisera give a positive reaction with the tumour cells, although allogeneic isoantisera consistently gave strongly positive reactions. The results are interpreted as showing that AAF‐induced rat mammary adenocarcinomas are deficient in tumour‐specific transplantation antigens.