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Positively charged groups at cell surfaces
Author(s) -
Weiss L.,
Bello J.,
Cudney Toni L.
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030613
Subject(s) - electrokinetic phenomena , reagent , sarcoma , electrophoresis , cell , chemistry , biophysics , biochemistry , biology , microbiology and biotechnology , pathology , medicine , organic chemistry
An attempt was made to identify positively charged amino‐groups at the electrokinetic surfaces of human and mouse erythrocytes, cultured cells derived from a human osteogenic sarcoma, and murine ascites sarcoma 37 cells. Following reactions of these cells with formaldehyde, 2,4,6‐trinitrobenzenesulfonic acid; 2‐chloro‐3,5‐dinitropyridine or 2‐chloro‐3,5‐dinitrobenzoic acid, no consistent change in their electrophoretic mobilities was observed. If a number of positively charged groups commonly associated with proteins had been present at the cellular electrokinetic surface, their reaction with the various reagents would have resulted in an increase in net surface negativity. We therefore conclude that these groups are not present at the surfaces of the cells studied.