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Comparative studies on the amount of DNA in cell nuclei of mouse skin after administration of carcinogenic and non‐carcinogenic substances
Author(s) -
Inui Naomichi,
Takayama Shozo
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030602
Subject(s) - croton oil , carcinogen , turpentine , epithelium , dna , papilloma , feulgen stain , hyperplasia , cell , pathology , chemistry , biology , microbiology and biotechnology , biochemistry , medicine , immunology , inflammation
The amount of DNA in cell nuclei was determined by microspectrophotometry on Feulgen‐stained preparations using basal cells from the back skin of mice after painting with 3‐methylcholanthrene (3‐MC), croton oil and turpentine, and from skin after a burn. In the hyperplastic epithelium of the skin painted with 3‐MC, the amount of DNA per cell nucleus was the same as that of normal skin but the mean amount of DNA in papilloma and atypical hyperplasia was slightly higher. However, no significant difference was found from that of the normal. In contrast to the above lesions, the amount of DNA in squamous cell carcinomas was elevated and the range of modal distribution was wider. In the hyperplastic epithelium induced by painting with croton oil or turpentine, the amount of DNA was also near the normal range. In the regenerating hyperplastic epithelium after a burn, the number of cells with an increased amount of DNA was higher for 2–5 days after the burn but no essential difference was observed from the normal.