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Polyoma virus replication in ascitic cells
Author(s) -
Nordenskjöld Bo
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030511
Subject(s) - virus , polyoma virus , antibody , virology , biology , hemagglutination , ascites , ehrlich ascites , ascitic fluid , immunology , tumor cells , cancer research , medicine , gastroenterology
Several ascitic tumors have been tested for polyoma sensitivity. Three tumors, one methylcholanthrene‐induced and two polyoma‐virus‐induced, were demonstrated to be sensitive to polyoma virus as judged by plaque titration, hemagglutination and fluorescent antibody assay. These determinants were correlated with the number of surviving cells and appearance of antiviral antibodies. The amount of virus produced and the number of infected cells found increased exponentially to reach a plateau about 50–100 hr post infection, after which the two respective counts either remained stationary or proceeded to fall. A reduction in virus recovery was paralleled by the appearance of antiviral antibodies in the serum. The total number of cells present in the peritoneal cavity remained stationary, or dropped after polyoma infection, suggesting the possibility that polyoma virus acted as an oncolytic agent. The high number of infected cells obtained at optimal times after polyoma exposure suggests the possibility that ascites tumors can be a useful routine tool for biochemical and other experiments where an abundant source of polyoma‐infected cells is needed.