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Immunologic studies on graffi‐virus‐infected spontaneous gross leukemia of akr mice
Author(s) -
Micheel B.,
Pasternak G.
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030509
Subject(s) - antigen , virology , biology , virus , leukemia , immunofluorescence , antibody , immunology
Spontaneous AKR Gross leukemias permanently produced Graffi virus if the cells had been repeatedly passaged in Graffi‐virus‐infected mice. Only one of 10 Graffi‐virus‐infected Gross leukemias investigated appeared to be immunologically indistinguishable from Graffi leukemias. Its cells contained the Graffi‐virus‐induced surface antigens as demonstrated by the phenomenon of tumor‐specific transplantation resistance in vivo and by immunofluorescence and absorption tests in vitro. The original leukemias transplanted independently did not contain these antigens. All other superinfected Gross leukemias were resistant to the rejection response of animals immunized against Graffi leukemia. Weak positive reactions in the immunofluorescence test indicated the presence of certain Graffi‐virus‐induced antigens at their cell surface. In addition, the cells absorbed virus‐neutralizing antibodies from Graffi‐immune sera. Absorbed sera still contained antibodies against surface antigens of Graffi target cells. It is suggested that Graffi leukemia cells as well as one of the Graffi‐virus‐infected Gross leukemias tested contain viral and virus‐induced new cellular antigens while the other infected Gross leukemias contain only viral antigens.