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Immunological study of leukemias in bald mice
Author(s) -
de Pirosky Rosa Rabinovich
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030404
Subject(s) - antiserum , titer , leukemia , antigen , spleen , hyperimmunization , biology , immunology , immunogen , virology , lymph , cytotoxic t cell , antibody , medicine , pathology , in vitro , biochemistry , monoclonal antibody
A study of the antigenic relationship between four leukemias in the BALB strain has been carried out. These included a spontaneous leukemia and three induced by different exogenous agents such as Sarcoma 180, 32 P, and human neoplastic tissue. Antisera were obtained by allogeneic hyperimmunization in Rockland inbred mice using fresh homogenates of BALB leukemic spleen and mesenteric lymph nodes. The four antisera with hemagglutinating titers between 1/1,280 and 1/5,120 and cytotoxic titers between 1/160 and 1/320, were absorbed by normal BALB cells until the titers fell below 1/10. These absorbed antisera possessed neutralizing activity against fresh leukemic cells, as demonstrated in tests with live challenges. However each antiserum proved to be active against all four leukemias while each of the leukemias could absorb the neutralizing activity of any antisera. These experiments only allowed the detection of one antigen in the four leukemias studied, no antigenic difference being observed among them by this technique. Taking into account the fact that the BALB strain of this laboratory has a spontaneous leukemia incidence of 15%, these results suggest that the exogenous agents which induced three of these leukemias acted by triggering a latent leukemogenic process.