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Pathogenesis of rous sarcoma virus in the chick embryo with particular reference to vascular lesions
Author(s) -
Coates H.,
Borsos T.,
Foard M.,
Bang F. B.
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030403
Subject(s) - rous sarcoma virus , embryo , biology , virus , chorioallantoic membrane , incubation , yolk sac , virology , lesion , incubation period , inoculation , andrology , pathology , immunology , medicine , biochemistry , microbiology and biotechnology
1. Growth of strains of Rous sarcoma virus on the chorio‐allantoic membrane continues throughout the embryo's development, with some decrease in the rate of growth at the end. Susceptibility of the chorio‐allantoic membrane, as measured by the total number of lesions following a standard inoculum, was greatest at 11 to 13 days of incubation. The proportion of vascular pocks of the membrane was however much higher in embryos inoculated at 7 or 8 days of incubation. 1 Growth of virus in the chick embryo liver after intravenous inoculation was also continuous throughout incubation. Age of susceptibility, again measured by number of lesions, was however greatest at 8 to 10 days. 2 Examination of vascular lesions in the living embryo, and study of whole mounts stained by periodic acid‐Schiff method as well as of histological sections, all indicated that the vascular lesion was at least predominantly a localized neoplasm of the vascular endothelium. The early presence of PAS‐positive cells in the developing pock was demonstrated, an observation hitherto limited to tissue culture. 4 The presence of antibody to Rous virus in the yolk of the embryo was correlated with a decrease of vascular lesions following inoculation of 13‐day embryos but not ofj I‐day embryos. 5 Following intravenous inoculation, virus was probably limited to the initial area of infection during the first few days, but viremia was irregularly demonstrable from 3 days on. 6 In the discussion it is emphasized that tumors of the embryo or the chicken provoked by the Rous virus are primarily tumors of tissues derived from the mesoderm. This includes the capacity to cause vascular lesions. The production of avion lymphomatosis may well be an exception, but since the lymphatic tissue has not been clearly demonstrated to be of ectodermal origin, this remains an open question. Virus growth and tissue culture ‘ transformation ’ do occur in a variety of tissues.