Premium
The effect of halogenated nucleosides on the sensitivity of a mouse lymphoma P388F TO X‐rays and methyl methanesulphonate
Author(s) -
Fox Margaret
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030308
Subject(s) - deoxyuridine , sensitization , alkylation , dna , lymphoma , cell culture , chemistry , carcinogen , dna synthesis , cytotoxic t cell , microbiology and biotechnology , biology , stereochemistry , cancer research , biochemistry , genetics , in vitro , immunology , catalysis
Abstract Populations of the P388F mouse lymphoma were sensitized to the effects of X‐rays by prolonged culture in non‐toxic doses of 5‐iodo‐2'‐deoxyuridine and 5‐bromo‐2'‐deoxyuridine. A marked decrease in the ability of analogue‐exposed cells to recover from fractionated doses of X‐rays was found. Thus the mechanism of sensitization may be related to inhibition of repair mechanisms. Replicate cultures of the same populations did not show an increase in sensitivity to the monofunctional alkylating agent methyl methanesulphonate (MMS), which suggests that damage by X‐rays and MMS may be by different mechanisms in the cell line. The results would also suggest that the alkylation of DNA by MMS is not the only cytotoxic effect of this drug.