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Friend virus‐induced transplantable tumors of C57Bl/6 origin containing unusually minute amounts of infectious virus
Author(s) -
Odaka Takeshi,
Ikawa Yoji
Publication year - 1968
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910030206
Subject(s) - virus , friend virus , ascites , neoplasm , spleen , virology , biology , rickettsia , murine leukemia virus , leukemia , tumor cells , pathology , immunology , cancer research , medicine
A transplantable solid tumor and its ascites variant were established from a graft of the enlarged spleen of a C57B1/6 mouse which had been infected with Friend virus shortly after birth. Repeated virus assays on these tumors were mostly negative. The virus content of the tumors was not more than one thousandth of that of the tumors which had been induced by the same virus in A and DDD mice. Under an electron microscope, the chance of observing viral particles in these tumor cells was extremely small. The inoculation of viable ascites tumor cells into allogeneic DDD mice induced neither tumor growth nor generalized leukemia, leaving specific resistance against the subsequent challenge of a histocompatible Friend virus‐induced tumor. A single intraperitoneal injection of 2.0 × 10 7 ascites cells sufficed to retard or suppress the growth of tumor cells used for the test challenge. The use of C57B1/6 mice for the study of Friend leukemia is discussed.