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Attempts to produce carcinogenesis in organ cultures of mouse prostate with polycyclic hydrocarbons
Author(s) -
Röller Manuela R.,
Heidelberger Charles
Publication year - 1967
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910020513
Subject(s) - anaplasia , stilbestrol , hyperplasia , carcinogen , carcinogenesis , benzanthracene , methylcholanthrene , prostate , pathology , biology , chemistry , hormone , medicine , endocrinology , diethylstilbestrol , cancer , biochemistry
Abstract Pieces of C3H mouse prostate were maintained in organ culture with Eagle's MEM Abbreviations used: 20‐(3)Methylcholanthrene, MCA; 1,2,3,4,‐dibenzanthracene, 1,2,3,4‐DBA; 1,2,5,6‐dibenzanthracene, 1,2,5,6‐DBA; 9,10‐dimethyl‐1,2‐benzanthracene, DMBA; minimal essential medium, MEM. medium and 10 % horse serum in the presence and absence ofpolycyclic hydrocarbons, stilbestrol, and testosterone. With 1,2,5,6‐dibenzanthracene, methylcholanthrene, and 9,10‐dimethyl‐1,2‐benzanthracene massive hyperplasia, anaplasia, abnormal mitoses, and invasion of pleomorphic epithelial cells through the basement membrane were observed. Similar observations were made, but to a lesser degree, with stilbestrol. With the controls there was moderate hyperplasia and little anaplasia, while testosterone promoted normal differentiation. With the non‐carcinogenic 1,2,3,4‐dibenzanthracene, considerable hyperplasia but little anaplasia was produced. In all cases the viabilities were good and comparable. Prostate pieces from organ cultures treated with the three carcinogenic hydrocarbons were implanted into 872 C3H mice under a variety of conditions, but no tumors were induced. Nevertheless, this system served as the foundation for the subsequent achievement of carcinogenesis in vitro.