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Autoradiographic analysis of the proliferation in vitro of six murine cell lines of non‐malignant or malignant origin
Author(s) -
Sawicki W.,
Kieler J.,
Briand P.
Publication year - 1967
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910020210
Subject(s) - mitosis , thymidine , cell culture , malignancy , in vitro , cell growth , dna synthesis , malignant cells , biology , tissue culture , microbiology and biotechnology , cell cycle , cell division , cell , pathology , immunology , cancer research , genetics , medicine , cancer
A high rate of cell growth in culture has often been thought to be indicative of malignancy. In the present work the generation time of six murine cell lines and the duration of their various mitotic and intermitotic phases were studied using thymidine‐6‐ 3 H and autoradiography. No definite correlation could be established between the growth kinetics and the tumor‐producing capacity of these cells, but cultures which were more than one year old showed a slightly shorter generation time and a somewhat shorter phase of DNA synthesis (ts) than 6‐ to 8‐month‐old cultures.