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The effect of Rous sarcoma virus (Schmidt‐Ruppin strain) on the chromosomes of human leucocytes in vitro
Author(s) -
Schendler Shirley,
Harris R. J. C.
Publication year - 1967
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910020206
Subject(s) - rous sarcoma virus , biology , in vitro , strain (injury) , mycoplasma , tissue culture , virus , embryo , mitosis , bromodeoxyuridine , virology , chromosome , microbiology and biotechnology , anatomy , genetics , cell growth , gene
Human leucocyte cultures have been incubated in vitro with Rous sarcoma virus of the Schmidt‐Ruppin strain (RSV(S‐R)). Mitosis appeared to be inhibited to a degree dependent on the virus concentration. However, and contrary to other reports, the incidence of chromosome breaks (6 in 612 metaphases examined) in such cultures did not significantly differ from that found in controls (3 in 729) and was less than that for cultures incubated with a microsome fraction from chick embryo tissue homogenate (10 in 262). 5‐Bromodeoxyuridine (BUDR) produced 17 breaks in the 77 metaphases examined (22%) and the Negroni mycoplasma (from leukaemic bone‐marrow) gave 21 breaks in the 70 cells analysed.