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The detection of intracellular adenovirus type 12 antigens by indirect immunoferritin technique
Author(s) -
Levinthal J. D.,
Cerottini J. C.,
AhmadZadeh C.,
Wicker R.
Publication year - 1967
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910020204
Subject(s) - antigen , hamster , antibody , immunofluorescence , fluorescein , ferritin , intracellular , digitonin , fixation (population genetics) , chemistry , microbiology and biotechnology , glutaraldehyde , biology , virology , membrane , immunology , biochemistry , chromatography , fluorescence , physics , quantum mechanics , gene
An indirect immunoferritin technique for the localization of intracellular antigens is described. By the use of a variety of specific hamster and rabbit sera followed by double‐labeled fluorescein‐ferritin antiglobulins, antigens induced by human adenovirus type 12 could be specifically marked by a reaction analogous to the indirect technique of immunofluorescence in infected and transformed hamster, human and monkey cells. The cellular plasma membrane was rendered permeable to antibody with a dilute solution of digitonin, and fixation in dilute formaldehyde, 0.5 %, preserved a reasonable degree of morphologic detail as well as antigenic activity. Whole cells were reacted in suspension with antibody followed by antiglobulin. The antigens “stained” by ferritin‐labeled antibody were then located in thin sections after post‐fixation and Epon embedding. It is concluded that use of the indirect technique should permit a more general utilization of ferritin‐labeled antibodies.