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Pituitary hormones and the metabolism of N ‐hydroxy‐ N ‐2‐fluorenylacetamide in female rats
Author(s) -
Shirasu Y.,
Grantham P. H.,
Weisburger J. H.
Publication year - 1967
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910020110
Subject(s) - endocrinology , medicine , urine , glucuronide , carcinogen , urinary system , chemistry , metabolism , excretion , hormone , biology , biochemistry
The transplantable functional pituitary tumor, Furth MtT/F4, enhances hepatoma formation by the carcinogen N‐hydroxy‐N‐2‐fluorenylacetamide (N‐OH‐FAA) in rats. Therefore the metabolism of a single intraperitoneal dose of N‐OH‐FAA‐9‐ 14 C was followed in groups of female Fischer rats which were fed either N‐OH‐FAA‐containing or control diets for 8 weeks, with or without MtT implantation. The MtT markedly increased rectal temperature, food and water intake, urinary volume and body, liver, and kidney weights. The carcinogen‐prefeeding alone did not affect the total percentage of radioactivity from labeled N‐OH‐FAA excreted in 24‐hour urine, but the proportion excreted as urinary glucosiduronic acids and as the glucuronide of N‐OH‐FAA was greater. MtT implantation had no significant effect on the urinary metabolites in the animals on a control diet. However, the combination of carcinogen‐prefeeding and MtT transplantation increased the urinary excretion of total radioactivity, the urinary glucosiduronic acids, and especially the glucuronide of N‐OH‐FAA. Maintenance of higher levels of N‐OH‐FAA, the altered endocrine situation and the increased metabolic rate may contribute to the enhancement of hepatoma formation in female rats implanted with MtT.