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Further studies on the membrane immunofluorescence reaction of Burkitt lymphoma cells
Author(s) -
Klein Eva,
Clifford Peter,
Klein George,
Hamberger CarlAxel
Publication year - 1967
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910020106
Subject(s) - lymphoma , bone marrow , immunofluorescence , medicine , antigen , pathology , lymph node , epstein–barr virus , leukemia , immunology , biopsy , antibody , burkitt's lymphoma , virus
The specificity of the membrane immunofluorescence reaction obtained upon the exposure of viable Burkitt lymphoma biopsy cells to the sera of certain Burkitt lymphoma patients (Klein et al., 1966 a, b) was studied by testing representative negative and positive sera against Burkitt lymphoma cells and normal bone marrow cells from the same donor, as well as against a panel of allogeneic normal lymph node cells and chronic myeloid leukemia cells from Swedish patients. Sera giving highly positive reactions with Burkitt lymphoma cells failed to react or reacted only exceptionally with normal bone marrow cells of the same donors or the allogeneic non‐Burkitt target cells. In contrast, an isoimmune serum from a multiply transfused donor gave highly positive reactions against both Burkitt and non‐Burkitt target cells. Taken together with previous results, these findings speak against the involvement of blood group or transplantation antigens, leaving two main explanations: (a) a tumor‐specific reaction, similar to the findings on virus‐induced mouse lymphomas: (b) preferential non‐specific binding to Burkitt lymphoma cell surfaces of some globulin fraction appearing in the serum of Burkitt lymphoma patients after chemotherapeutically induced regression.