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Covalent binding of 4‐dimethylaminophenylazo‐ 3 H‐benzene (butter yellow) metabolites with liver ribosomal RNA: The dissociation of the binding mechanism from the orotic acid incorporating system
Author(s) -
Roberts J. J.,
Warwick G. P.
Publication year - 1966
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910010606
Subject(s) - orotic acid , rna , biochemistry , metabolism , chemistry , metabolite , microbiology and biotechnology , biology , stereochemistry , gene
1. No overall correlation between the binding of 3 H‐labelled metabolites of 4‐dimethylaminophenylazobenzene (DAB) to the r‐RNA of liver, spleen and kidney and the incorporation of orotic acid‐ 14 C into the same fractions after the same time was observed. 2. While actinomycin D inhibited the incorporation of orotic acid‐ 14 C into liver r‐RNA it did not cause a similar depression in the binding of 3 H‐labelled metabolites of DAB to r‐RNA. 3. Actinomycin D did not affect the overall metabolism of DAB as judged by the binding of 3 H‐labelled metabolites to liver cytoplasmic and nuclear protein fractions. 4. These results suggest that the association of radioactivity with liver r‐RNA following injection of DAB‐ 3 H is mediated by reactions independent of the orotic acid incorporation mechanism. They also confirm the earlier findings that reaction with liver r‐RNA is particularly favoured compared to that with r‐RNA from other sites.