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Further observations on in vitro cytopathic effects associated with murine leukemia virus infection
Author(s) -
Tyndall R. L.,
Teeter E.,
Otten J. A.,
Bowles N. D.,
Vidrine J. G.,
Upton A. C.,
Walburg H. E.
Publication year - 1966
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910010605
Subject(s) - biology , leukemia , virology , cytopathic effect , in vitro , virus , cell culture , tissue culture , immunology , biochemistry , genetics
The previously reported cytopathic effects (CPE) in an established cell culture chronically infected with the Rauscher murine leukemia virus have been investigated in more detail. Initiation of CPE and morphologic transformation was observed in newly infected cultures. Suppression of CPE and transformation by NCTC 109 medium containing 25% human serum was also found to be reproducible in newly infected cultures. Adult human serum, human cord serum, fetal calf serum and fetuin had a suppressive effect on the CPE and transformation. Serological tests to detect non‐leukemogenic mouse viruses in the transformed cells were negative. Infected, trans‐formed cells were highly tumorigenic when inoculated subcutaneously into BALB/c mice. The resultant tumors were classified as myxofibrosarcomas. Approximately 50% of such tumor‐bearing mice also developed leukemia. In contrast, control uninfected cells under similar conditions were less tumorigenic and produced spindle‐cell sarcomas. None of the mice injected with control uninfected cells developed leukemia. The tumori‐genicity of both the infected and uninfected cell cultures increased when the cells were cultured on 4 × BME medium containing 10% calf serum.