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Biologic characterization of gross lymphoma homogenates concentrated by density gradient and differential centrifugation
Author(s) -
Wahren Britta
Publication year - 1966
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910010206
Subject(s) - lymphoma , differential centrifugation , centrifugation , antibody , antigen , biology , immunology , microbiology and biotechnology , pathology , chemistry , medicine , biochemistry
Fractions of Gross lymphoma homogenates were prepared by density gradient centrifugation or by differential centrifugation. Primary lymphomas developed in 80% of newborn mice inoculated with pellets obtained after differential centrifugation and in 48% of mice inoculated with fractions of Gross lymphoma homogenate with a density of 1.16–1.18. Such fractions induced a relative transplantation resistance as measured by a decrease in the frequency of tumor takes and a prolonged latency period when isogenic Gross lymphoma cells were inoculated into immunized mice. Sera obtained from mice immunized with fractions of density 1.16–1.18 from Gross lymphoma homogenate were shown to contain cytotoxic antibodies active against Gross lymphoma cells. The results indicate that viral particles are responsible for the induction of antibodies against the Gross lymphoma antigen, either directly by sharing antigenic properties with the surface of the lymphoma cells from which they are derived, or indirectly by infection and transformation of susceptible cells.