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Leukemogenic activity of centrifugates from irradiated mouse thymus and bone marrow
Author(s) -
HaranGhera Nechama
Publication year - 1966
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910010111
Subject(s) - bone marrow , irradiation , lymphoma , spleen , biology , capsule , renal capsule , incidence (geometry) , kidney , andrology , pathology , immunology , medicine , endocrinology , botany , physics , nuclear physics , optics
The leukemogenic activity of centrifugates from radiation‐induced lymphomas and irradiated tissues of C57BL mice was tested by injecting these directly into 5 to 7‐day‐old thymus implants beneath the kidney capsule in thymectomized irradiated hosts of the same strain. A series of 12 centrifugates from radiation‐induced lymphomas were tested for leukemogenic activity. Of these, six were found to be active, inducing about 14 per cent lymphomas at an average latent period of 166 days. Centrifugates from thymus plus bone marrow taken from irradiated donor mice 5 or 10 days after completion of irradiation demonstrated leukemic activity—an incidence rate of up to 30 per cent. Centrifugates of irradiated tissues taken 20, 30, 45 and 60 days after irradiation showed only borderline activity. Thymic tissue, taken from the same irradiated donor mice, transplanted beneath the kidney capsule of normal hosts, revealed neoplastic transformation only when taken from donors 30 days after completion of the irradiation. Urethane was found to increase the leukemogenic activity of the centrifugate. Centrifugates prepared from tissues taken from mice treated concurrently with X‐rays and urethane 10 days after completion of treatment yielded 75 per cent lymphoma incidence, with a marked decrease in the average latent period. The first passage of this active centrifugate similarly revealed an 80 per cent lymphoma incidence, with a short latent period. Centrifugates of tissues taken 20, 30 and 45 days after irradiation and urethane treatment showed no activity. It is concluded that irradiation causes the “release” of a transmissible leukemogenic agent in C57BL mice, and that urethane potentiates the activity of this agent.