Premium
NEDD9 promotes lung cancer metastasis through epithelial–mesenchymal transition
Author(s) -
Jin Yujuan,
Li Fei,
Zheng Chao,
Wang Ye,
Fang Zhaoyuan,
Guo Chenchen,
Wang Xujun,
Liu Hongyan,
Deng Lei,
Li Cheng,
Wang Hongda,
Chen Haiquan,
Feng Yan,
Ji Hongbin
Publication year - 2013
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.28568
Subject(s) - metastasis , epithelial–mesenchymal transition , cancer research , lung cancer , cancer , gene knockdown , biology , melanoma , cell migration , pathology , medicine , cell , cell culture , genetics
Metastasis is the major cause for high mortality of lung cancer with the underlying mechanisms poorly understood. The scaffolding protein neural precursor cell expressed, developmentally down‐regulated 9 (NEDD9) has been identified as a pro‐metastasis gene in several types of cancers including melanoma and breast cancer. However, the exact role and related mechanism of NEDD9 in regulating lung cancer metastasis still remain largely unknown. Here, we demonstrate that NEDD9 knockdown significantly inhibits migration, invasion and metastasis of lung cancer cells in vitro and in vivo . The pro‐metastasis role of Nedd9 in lung cancer is further supported by studies in mice models of spontaneous cancer metastasis. Moreover, we find that NEDD9 promotes lung cancer cell migration and invasion through the induction of epithelial–mesenchymal transition (EMT) potentially via focal adhesion kinase activation. More importantly, NEDD9 expression inversely correlates with E‐cadherin expression in human lung cancer specimens, consistent with the findings from in vitro studies. Taken together, this study highlights that NEDD9 is an important mediator promotes lung cancer metastasis via EMT.