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Evaluation of a multiplex panel of immune‐related markers in cervical secretions: A methodologic study
Author(s) -
Koshiol Jill,
Sklavos Martha,
Wentzensen Nicolas,
Kemp Troy,
Schiffman Mark,
Dunn S. Terence,
Wang Sophia S.,
Walker Joan L.,
Safaeian Mahboobeh,
Zuna Rosemary E.,
Hildesheim Allan,
Pfeiffer Ruth M.,
Pinto Ligia A.
Publication year - 2013
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.28354
Subject(s) - cervical intraepithelial neoplasia , medicine , odds ratio , cervical cancer , confidence interval , cervix , gastroenterology , hpv infection , oncology , immunology , cancer
Although persistent carcinogenic human papillomavirus (HPV) infection is necessary for cervical carcinogenesis, the cofactors involved in HPV persistence and disease progression are poorly understood. Chronic cervical inflammation may increase risk, but few studies have measured immune markers (cytokines, chemokines and soluble receptors) in cervical secretions. We evaluated the performance of 74 multiplexed, bead‐based immune markers in cervical secretions from three groups of women with biopsy evaluation of cervical intraepithelial neoplasia (CIN), ( i ) 25% detectability and >80% interclass correlation coefficients (ICCs) acceptable for epidemiologic studies. Within‐batch coefficients of variation (CVs) of ≥25% indicated room for assay improvement. Secondarily, we explored associations between marker levels and CIN/HPV status adjusted for matching variables, assay batch, age and number of sexual partners. Sixty‐two markers (84%) had >25% detectability and ICCs > 80%. Of those, 53 (85%) had CVs < 25%. Using these preliminary data, we found that HPV positivity was associated with increased eotaxin‐1 [odds ratio (OR): 15.63, 95% confidence interval (CI): 1.26–200.00] and G‐CSF (OR: 12.99, 95% CI: 1.10–142.86) among CIN‐negative women. There was suggestive evidence that higher chemoattractant marker levels were associated with CIN2/3 ( e.g ., MIP‐1delta, OR: 4.48, 95% CI: 0.87–23.04 versus