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Oncogenic mutations in extramammary Paget's disease and their clinical relevance
Author(s) -
Kang Zhihua,
Xu Feng,
Zhang Qiaoan,
Wu Zhiyuan,
Zhang Xinju,
Xu Jinhua,
Luo Yan,
Guan Ming
Publication year - 2012
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.27738
Subject(s) - hras , neuroblastoma ras viral oncogene homolog , kras , akt1 , cancer research , cdh1 , extramammary paget's disease , mutation , pathogenesis , biology , cancer , gene , pi3k/akt/mtor pathway , genetics , medicine , disease , pathology , signal transduction , cadherin , immunology , cell
Extramammary Paget's disease (EMPD) is a rare cutaneous malignant neoplasm. The genetic alterations underlying its pathogenesis have less been described. Therefore, we analyzed the possible mutations in the KRAS , HRAS , NRAS , BRAF , ARAF , RAF1 , PIK3CA , AKT1 , CTNNB1 and APC genes as well as methylation and expression of CDH1 in 144 EMPD cases and 42 matched normal skin tissues. A distinct mutation profile was identified in EMPDs with 27 (19%) cases mutant for RAS and RAF genes and 50 (35%) cases harboring oncogenic mutations in PIK3CA and AKT1 . Moreover, a mutually exclusive pattern was observed in the genetic variants in these two signaling pathways. No mutation was detected in CTNNB1 and APC genes. High prevalence of low expression and hypermethylation of CDH1 gene was detected in 33 and 48% of the EMPD cases, respectively. Furthermore, PIK3CA and AKT1 mutations were significantly correlated with CDH1 hypermethylation which could explain why the majority of EMPD cases with mutant PIK3CA and AKT1 were invasive. Our study demonstrates that genetic variants associated with constitutive activation of RAS/RAF and PI3K/AKT pathways are involved in the pathogenesis of EMPD. This may represent novel therapeutic targets for this skin cancer.