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High‐risk human papillomavirus in oral squamous cell carcinoma of young patients
Author(s) -
Kaminagakura Estela,
Villa Luísa Lina,
Andreoli Maria Antonieta,
Sobrinho João Simão,
Vartanian José Guilherme,
Soares Fernando Augusto,
Nishimoto Inês Nobuko,
Rocha Rafael,
Kowalski Luiz Paulo
Publication year - 2011
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.26185
Subject(s) - human papillomavirus , polymerase chain reaction , carcinogenesis , risk factor , medicine , typing , immunostaining , tissue microarray , basal cell , carcinoma , in situ hybridization , cancer , oncology , gastroenterology , pathology , immunohistochemistry , biology , gene , gene expression , genetics
The aim of this study was to investigate a possible relation between oral squamous cell carcinoma (SCC), the presence of high‐risk human papillomavirus (HR‐HPV) DNA and p16 expression in young patients. Paraffin‐embedded tumor blocks from 47 oral SCC of young (≤40‐year old) patients were evaluated. The presence of HPV DNA in tumor specimens was analyzed by polymerase chain reaction (PCR) using GP5+/GP6+ generic primers (L1 region) followed by dot blot hybridization for HPV typing. When necessary, the HPV16 positivity was confirmed by PCR HPV16 E7‐specific primers. Cases involving young patients were compared with 67 oral SCC from patients ≥50‐year old (controls). Demographic and clinical data were collected to analyze patient outcomes. p16 ink4 expression was evaluated by immunostaining of tissue microarrays. HPV16 was detected in 22 (19.2%) cases; 15 (68.2%) young and 7 (31.8%) control patients, a statistically significant difference ( p = 0.01). In 1 (1.7%) young group specimen, HPV DNA 16 and 18 was detected. p16 expression was observed in 11 (25.6%) cases from the young group and in 11 (19.6%) controls ( p = 0.48). Association between HPV and p16 was verified, and it was statistically significant ( p = 0.002). The higher prevalence of high‐risk HPV types, especially HPV16, may be a contributing factor to oral carcinogenesis in younger individuals.