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Poster presentations (PP01–PP47)
Author(s) -
Zhu, M,
Wang, N,
Tsao, SW,
Tong, Y,
Feng, Y
Publication year - 2011
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.25877
Subject(s) - medicine
Poster Presentations: no. PP15aThis FREE journal suppl. entitled: Supplement: Abstracts of the Excellence in Oncology 2010 Conference 18–20 November 2010, Hilton Hotel, Athens, GreeceConference Theme: Cutting Edge Findings Into Clinical PracticeINTRODUCTION: Hepatocellular carcinoma is one of the most malignant cancers with dismal outcomes and the therapeutic strategies remain poor and far from developed. 3alpha,7beta-Dihydroxy-5beta-cholan-24-oic Acid N-(2-Sulfoethyl) amide (Tauroursodeoxycholic Acid, TUDCA) is currently in frequent used for protect the hepatocyte in several kinds of liver disorders including liver cancer. MicroRNA-21 (miR-21) overexpression was reported correlated with the high invasive property of cancer cells. PURPOSE: To profile the microRNA expression alteration in hepatocarcinoma cells with treatment of TUDCA and observe the effect of TUDCA in the invasion and metastasis of hepatocellular carcinoma. Material: Human hepatocellular carcinoma cell line with high metastatic property MHCC97-L. METHODS: We developed a sensitive microRNA on-chip array to detect the miRNAs expression profiling in MHCC97-L cells with or without TUDCA exposure. A quantitative real-time PCR method was introduced to validate the on-chip result. The cell migration was detected by wound healing the invasion chamber assay. Inhibition of miR-21 was conducted by introducing an antisense oligonucleotide complementary to the miRNA. RESULTS: Three microRNAs, let-7f, miR-21 and miR-23a were up-regulated in MHCC97-L with 100 lM TUDCA exposure for 48 hr. The result of the qPCR validates the increase of miR-21 expression in MHCC97-L cells treated with TUDCA comparing with the control. Increased cell migration was observed in MHCC97-L cells with 100 lM TUDCA exposure. Addition of miR-21 inhibitor attenuates the effect of TUDCA on MHCC97-L cell migration.link_to_OA_fulltextExcellence in Oncology: Cutting Edge Findings Into Clinical Practice, Athens, Greece, 18-20 November 2010. In International Journal of Cancer, 2011, v. 128 suppl. 1, p. 39, abstract no. PP15