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Directed in vivo angiogenesis assay and the study of systemic neoangiogenesis in cancer
Author(s) -
Napoli Claudio,
Giordano Antonio,
Casamassimi Amelia,
Pentimalli Francesca,
Ignarro Louis J.,
De Nigris Filomena
Publication year - 2011
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.25743
Subject(s) - angiogenesis , in vivo , metastasis , medicine , neovascularization , cancer research , ex vivo , cancer , biology , microbiology and biotechnology
Abstract Targeting neoangiogenesis is a well‐established anticancer strategy, however, one of the major problems in angiogenesis research, both at the basic and applied levels, remains the development of suitable in vivo methods for assessing and quantifying the systemic angiogenic response. Therefore, there is an urgent need to adopt technically simple and reproducible methodologies which allow to easily quantify neoangiogenesis independently of morphological parameters. Recently, a reproducible and quantitative method was developed, the directed in vivo angiogenesis assay (DIVAA) consisting of the subcutaneous implantation of surgical grade silicone cylinders closed at one end, called angioreactors, into the dorsal flanks of nude mice. In the past few years, DIVAA has been successfully used in evaluating the inhibition and or enhancement of systemic perturbation of angiogenesis by several molecules. Thus, DIVAA studies systemic angiogenesis and its therapeutic modulation associated to cancer progression and metastasis.