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Intraepithelial p63‐dependent expression of distinct components of cell adhesion complexes in normal esophageal mucosa and squamous cell carcinoma
Author(s) -
Thépot Amélie,
Hautefeuille Agnès,
Cros MariePierre,
AbediArdekani Behnoush,
Pétré Aurélia,
Damour Odile,
Krutovskikh Vladimir,
Hainaut Pierre
Publication year - 2010
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.25221
Subject(s) - carcinogenesis , adherens junction , biology , cell adhesion , microbiology and biotechnology , cell , cell adhesion molecule , downregulation and upregulation , cancer research , cadherin , cancer , gene , genetics
TP63 gene is a member of TP53 tumor suppressor gene family that encodes several protein isoforms involved in the process of epithelial stratification and in epithelial‐mesenchyme interactions. TP63 is amplified in a significant proportion of squamous cell carcinoma of the esophagus (ESCC), resulting in the hyper‐expression of ΔNp63 as the major p63 isoform. To better understand the contribution of this high expression to tumorigenesis, we have analyzed the impact of intraepithelial p63 expression on the expression of cell adhesion complexes in normal esophagus and in ESCC cell lines. Cells expressing p63 showed an adhesion pattern characterized by lack of tight junctions and presence of adherens junctions. Cell differentiation was accompanied by a decrease in p63 and by a shift to adhesion patterns involving tight junctions. Silencing of p63 mRNA in ESCC cell lines resulted in a similar shift, characterized by increased expression of component of tight junctions, decreased cell‐to‐cell communication and downregulation of cell proliferation. These results indicate that ΔNp63 may contribute to esophageal squamous carcinogenesis by maintaining cell adhesion patterns compatible with cell proliferation.