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Type‐specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia
Author(s) -
Ho ChihMing,
Lee BorHeng,
Chang ShwuFen,
Chien TsaiYen,
Huang ShihHung,
Yan ChiuCho,
Cheng WenFang
Publication year - 2010
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.25078
Subject(s) - cervical intraepithelial neoplasia , genotyping , messenger rna , biomarker , dysplasia , real time polymerase chain reaction , rna , cervical cancer , cytology , oncogene , medicine , cancer , gene , genotype , biology , pathology , cell cycle , genetics
This study aimed to evaluate whether quantitation of high‐risk human papillomavirus (HR‐HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11/GP6+ PCR for HPV DNA amplification, followed by HPV genotype‐specific hybridization with on a gene chip. E6 type‐specific PCR was used to validate multiple infections. Quantitative real‐time reverse transcriptase (QRT‐PCR) and real‐time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid‐based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA‐positive cases was lower in normal women and