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The helix‐loop‐helix Id‐1 inhibits PSA expression in prostate cancer cells
Author(s) -
Zielinski Anne J.,
Fong Sylvia,
Allison Juanita,
Kawahara Misako,
Coppe JeanPhilippe,
Feiler Heidi,
Lee Nancy M.,
Desprez PierreYves
Publication year - 2009
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.24811
Subject(s) - lncap , prostate cancer , cancer research , biology , prostate , gene expression , microarray analysis techniques , prostate specific antigen , cancer , gene , pca3 , microbiology and biotechnology , genetics
The inhibitor of basic helix‐loop‐helix transcription factors, Id‐1, is an important gene whose expression increases during prostate cancer progression and that upregulates proliferation, migration and invasion. We used microarray analysis to identify the downstream genes whose transcriptional expression is modulated by Id‐1 protein. We compared gene expression in control LNCaP cells and Id‐1‐transduced LNCaP cells, which become significantly more aggressive after Id‐1 overexpression, thus mimicking the high levels of Id‐1 detected in metastatic cell lines. We used the Affy HTA U133A Expression Arrays with 45,000 probe sets representing more than 39,000 transcripts. We found that one of the most significantly downregulated genes on Id‐1 expression was kallikrein 3 [also called prostate specific antigen (PSA)], the most commonly used biomarker of prostate cancer. Here, we show that the reduction in PSA mRNA and protein expression associated with high‐grade prostate cancers, which generally express high levels of Id‐1, could be the consequence of Id‐1 overexpression.